NYONE® Cell Imager
NYONE® is a compact, automated cell imager, ideal for small to medium throughput labs. NyONE combines brightfield and optional fluorescence microscopy and provides the highest image quality in high-throughput settings. It is an ideal system for single cell cloning to confirm monoclonality, for fast cell imaging and cell counting with viability, and many other applications utilizing automated fluorescence microscopy.
NYONE® FEATURES
NYONE runs on the same software platform as CELLAVISTA 4®, supporting the same applications.
Performs automated, fast and non-invasive brightfield and fluorescence imaging with multi-parameter image analysis
Brightfield imaging and optional 3 or 4 fluorescence channels
Very fast scanning: complete scan of a 96-well microplate at 10x magnification in 5-6 minutes
Achieves high image quality using specifically designed optic and laser autofocus mechanisms
Parallel image processing
Intuitive software with comprehensive graphical output
Supports microplates of the SBS standard plate formats (384, 96, 48, 24, 12, 6), dishes, T 25 flasks and microscope slides.
Adherent and suspension cells
Complete documentation of cellular growth
Excellent well edge illumination of microplates
Perfect image stitching
Can be accompanied with Synentec’s SyBOT-800 automated system or linked to robotic systems from companies like Hamilton, Tecan, Perkin Elmer, Beckman Coulter, PAA, or ThermoFisher.
NyONE® MODELS
NYONE CLD HIGH END®
NYONE CLD HIGH END is equipped with 3 different lenses, achieving resolutions down to 360 nm/pixel, 3-4 excitation sources (UV to RED) and up to 6 different emission filters. All operating procedures are fully automated and can be embedded into a completely automated environment. In combination with our versatile YT-software a wide range of cellular assays is feasible.
NYONE SCIENTIFIC®
NYONE SCIENTIFIC is a brand new model with a highly sensitive 16-bit sCMOS camera and high numeric aperture lenses for measuring wider ranges of signal intensities, higher dynamic range, and a better signal to background ratio.
Downloads
Synentec_TechInfo_8pp_CLD.pdfTechnical Information
CLD Line
(2.5 MB)
Downloads
Synentec_TechInfo_8pp_Scientific.pdfTechnical Information
SCIENTIFIC Line
(2.5 MB)
NyONE® APPLICATIONS
NYONE® is a very versatile system that can be used for dozens of applications in cell line development, basic and translational research, and preclinical drug development.
The list of applications for NyONE include:
Trypan Blue Cell Viability Assays - analyze 96 samples in less than 3 minutes with sample volumes as little as 20 µL
Single Cell Cloning (SCC) and Fluorescence Activated Single Cell Cloning (FASCC)
High-Throughput Cell Counting and Cell Proliferation Monitoring
Monitoring Gene Transfection Efficiency
Cell Confluence Monitoring
High-Throughput Automated Cell Migration and Wound Healing Assays
Stem Cells, iPS Cell Characterization and Monitoring
Cell Apoptosis (Annexin V, JC-1, caspases)
DNA Damage
Nuclei Staining
Cell Cycle and Mitosis Analysis
CD Marker Analyses
Antibody and ADC Internalization Assays
Viral Plaque Assay and Vaccine Development
siRNA Detection
Oxidative Stress (ROS)
IgG and Glycan Quantification (in combination with PAIA plates)
ImmunoCytoChemistry (ICC/IHC)
3D Culture Assays
For a complete list of applications and technical notes, please visit Synentec's website.
High-throughput trypan blue dye exclusion assay for measuring cell counts and viability
a widely used technique to determine cell numbers and viability
very fast cell counting- 96 samples in 3 minutes!!!
small sample volumes, as low as 20 µL
using standard 96-well or 96-well half-area microplates
accurate and reproducible data
very low cost per sample
Downloads
TRYPAN BLUE APPLICATION NOTES.pdfTrypan Blue Viability Assay
Application Notes
(360 MB)
Single Cell Cloning - Proof Of Monoclonality
Single cell cloning (SCC) represents a crucial step in cell line development. The aim of SCC is to identify wells where a single cell was seeded and to monitor its growth rate. To prove monoclonality for regulatory approval, it is essential to have a robust and reliable imaging system. CELLAVISTA 4 and NYONE automated cell imagers are well established in the field of cell line development. They combine robustness and reliability with a high image resolution (below 1 micron per pixel) to determine the clonality of a cell line.
Downloads
automating_the_proof_of_monoclonality_an-b132_xviii_05_.pdfAutomating the Proof of Monoclonality
Technical Notes
(1.9 MB)
Single Cell Cloning (SCC) using brightfield imaging
Our systems scan the whole wells of a microplate in bright field at high resolution after seeding the cells.
Benefits:
rapid cell cloning
no area in the well is missed
day-by-day automatable measurement
all colonies are recognized by our software
tracking back to the first day to demonstrate monoclonality
works with both adherent and suspension cells
automatic generation of a clone gallery
This provides a proof that your colony in fact grows from a single cell, or grows from a cell doublet.
Downloads
single_cell_cloning__tn-b002-xvii-08_ (1).pdfSingle Cell Cloning
Technical Notes
(2.2 MB)
FASCC (Fluorescence Activated Single Cell Cloning)
Ghost cells represent one of the main challenges in cell line development. Such undetected cells usually lie on the well edge. They are sometimes missed because most imaging systems for CLD are equipped with low resolution optics (2 microns per pixel), and only have a white light (brightfield) source.
This lack of resolution causes a lack of information within the image, and therefore it is hardly possible to distinguish cells from debris, or well edge artefacts. Because of this lack of precision, a correct statement about monoclonality is difficult.
Adding fluorescence enables the detection of these cells in a reliable way, even at the well edge! SYNENTEC has developed a unique method called FASCC to overcome all disadvantages of classic white light single cell cloning. FASCC provides a fully automated screening of your microplates by using the advantage of short term, non-toxic, and animal-free cell staining (e.g., CellTracker Green, Calcein AM). This unique tool presents a fast proof of monoclonality without guesses or doubts!
FASCC-Workflow
a fast, low resolved (4x lens) high speed scanning mode (pre-scan) detect the fluorescence signal of e.g. Calcein-AM or Cell Tracker® stained cells
the embedded image analysis tool excludes all wells seeded with more than just one cell or any number of cell-count the user sets up
in the highly resolved Nanoview-scan all pre-scan results will be picked up and will be proceed by centering all single cells right in the middle of a single image (benefit: no cells on stitching boarder and fast scan despite high resolution)
over expansion-period confluence measurement or suspension cell count with automated colony-detection can be performed to track cell proliferation
Downloads
fascc__tn-b123-xvii-12_ (4).pdfFluorescence Activated Single Cell Cloning
(FASCC)
Technical Notes
(2.8 MB)
VIROLOGICAL RESEARCH AND Vaccine DEVELOPMENT
SYNENTEC's automated imagers are frequently used in virology and vaccine development (Influenza, Noro, SARS-CoV-2, Malaria, HIV, etc.) for human and veterinary vaccines. The high-throughput imaging system NYONE® accelerates your work and supports the following assays:
Viral titer quantification & infectivity determination
Label-free plaque assay
Endpoint dilution assays (TCID50)
Plaque reduction neutralization test (PRNT) (Virus neutralization assay)
Focus forming assay (FFA)
Immunofluorescence foci assays (IFA)
Measurement of host cell viability & viral cytopathic effects (Cell confluence, Live/Dead, Apoptosis...)
Viral Pathogenesis (morphological change in infected host cells)
In vitro immune responses to vaccines
Vaccine vector testing in transduction efficiency
Immunologic in vitro studies
Reverse vaccinology (ELISpot and FluoroSpot)
Please visit SYNENTEC’s website for more detailed information about NYONE in virology and vaccine development.
PRECLINICAL DRUG TESTING
The development of new drugs and biopharmaceuticals requires a precise experimental implementation. All steps to reach this goal involve time-consuming and labor-intensive laboratory testing. The automation of this process increases the throughput and quality of data, minimizes technician’s errors, and significantly increases the productivity of the lab.
SYNENTEC actively supports your daily laboratory work with their fully automated high-throughput cell imagers. Applications such as cell counting with viability, proliferation studies that help to determine drug sensitivity and resistance profiles, cell cycle and apoptosis monitoring, cell toxicity and DNA damage analyses, 3D cell culture assays, and surface marker analysis are only a fraction of the applications in which SYNENTEC’s versatile imagers provide an extremely valuable service to drug testing laboratories.
Downloads
Synentec_DrugTargetHandout_8.5x11 (1).pdfPreclinical Drug Testing
Brochure
(2.8 MB)
AUTOMATED WOUND HEALING ASSAY
Cell migration assays are very important tools to study the invasion processes and metastasis of cancer cells. They are also used in arthritis and osteoporosis research. The simplest approach to monitoring a cell migration is the so-called “scratch” assay. A pipette tip is used to scratch or “wound” in a confluent cell monolayer. Then a microscope is used to observe cells filling in or “repairing” the wound.
SYNENTEC in collaboration with the team led by Prof. Susanne Sebens from the Institute for Experimental Cancer Research, CAU + UKSH Kiel, Germany, developed the fastest and most flexible automated wound healing application. This wound healing assay features a high-throughput analysis using the NYONE® Scientific automated cell imager and SYNENTEC's YT®-Software.
This wound healing method is described in this YouTube video.
Downloads
woundhealing__an-b003-xvii-07_.pdfWound Healing Assay
Application Note
(5.7 MB)
SYNENTEC GmbH was founded in 2005 by engineers and partners Stefan Hummel and Matthias Pirsch. Their headquarters are located in Elmshorn, near Hamburg, Germany. SYNENTEC is a team of engineers, software developers, and biologists. All systems are designed, developed, and manufactured in house. CELLAVISTA, a high throughput fully automated cell imager, was launched in June 2007 and has been continuously developed ever since. NyONE, the compact automated cell imager is produced since 2014. More information about SYNENTEC, their imaging systems, applications, including downloadable material is available at www.synentec.com.